Yield of Urinary Pathogens and their Sensitivity Pattern at Paediatric Tertiary Care Facility, Karachi
Objective: To determine the yield of common pathogens isolated from urine specimens and their sensitivity pattern in children at National Institute of Child Health (NICH), Karachi.
Methods: This was a retrospective descriptive study conducted in the department of paediatric neph- rology and pathology, National Institute of Child Health over a period of two years, from January 2014 to December 2015. All urine samples received in our laboratory from children admitted to nephrology and other departments during the study period were processed for culture and sensitivity. All samples were inoculated with cystine lactose electrolyte deficient (CLED) agar plates and biochemical charac- teristics of the specific pathogen were identified on biochemical tubes. The plates and tubes were in- cubated for 24 hours at 37ºC and growth of more than 105 CFU was considered as significant. These colonies were identified by morphology, lactose fermentation, Gram stain and biochemical tests. Anti- biotic sensitivity was performed by Kirby-Bauer method as per CLSI (Clinical and Laboratory Standards Institute) guidelines and sensitivity was recorded after 24 hours' incubation at 37ºC.
Results: Out of 4107 samples processed, 1442 were positive giving a yield of 35% with 66% in girls and 34% in boys. E. coli was the most common pathogen (55%), followed by Pseudomonas (13.6%), Klebsiella (13,5%) and Candida albicans (11%). Majority of pathogens were sensitive to piperacillin- tazobactam (80-100%). Quinolones and aminoglycosides sensitivity was found in 65 to 85% and in 65 to 90% cases, respectively. Most of the pathogens (75%) were resistant to 3rd generation cepha- losporins except Citrobacter species.
Conclusion: A high yield of common pathogens in urine samples and high resistance to common antibiotics was observed.