FAREEDA ISLAM, SHAMIM S M, IQBAL AHSUN.

ABSTRACT

Doxorubicin an anthracycline antibiotic & antitumour agent is one of the highly effective antineoplastic drug for the treatment of several different human cancers. The clinical use of this drug is limited by its cardiotoxicity including dose related arrhythmias and often irreversible add from discussion cardiomyopathy. The inotropic and chronotropic activity of different doses of doxorubicin was first evaluated and after determining the fixed effective dose of doxorubicin, the beta receptor activity of doxorubicin was evaluated. The effect of propranolol on heart in presence of doxorubicin was evaluated. Thirty male rabbits weighing 1000 - 1500 grams were used. Drugs used were Doxorubicin and Propranolol. The actions of drugs were studied on the isolated heart of these animals. Krebs ringer buffered physiological solution was prepared and used. To perform the experiment complete set of Lagendroff`s apparatus was used. Recording was done on graph paper. Other accessories used were isometric transducer, thermostat oxygen, one ml graduated insulin syringe (to inject the test samples). Pate of contraction of heart and amplitude of contraction was measured (in centimeters) from baseline. Results were calculated statistically on S.P.S.S. We have evaluated the physiological interactions of Doxourbicin with Adrenaline (agonist) in the first part of the study and in this study the interaction with antagonist (Propranolol) has been studied to evaluate the effects leading to up or down regulation of b receptors.

For Full text contact to:
Prof. Dr. Farah Mansuri
editor@annals-ashkmdc.org

Department of Pharmacy, Hamdard University, Karachi *
Professor, Department of Pharmacology Baqai Medical College, Karachi *
Department of Anaesthesiology Karachi Medical & Dental College and Abbasi Shaheed Hospital, Karachi *