*RESHMA FAROOQ, **DARAKHSHAN HALEEM, ***TABASSUM MEHBOOB, ****M.A. HALEEM

ABSTRACT

Objective:Aim of this study was to determine the effect of diazepam on serum electrolytes (sodium, potassium, calcium, and magnesium), osmolarity and anxiolysis of 2hrs restraint stressed rats.

Method: Male Albino Wistar rats were used in experiment. Test animals were injected diazepam intraperitoneal and control deionized water. After thirty minutes of injection control and test animals were further divided into restrained and unrestrained groups. Animals of unrestrained group were kept in home cages, while restrained grouped animals were restrained by immobilizing for two hours(2hrs) on wire grids. Next day light-dark box activity was monitored for 5 minutes. Rats were decapitated. Blood was collected and serum separated for analysis of electrolytes.

Results: Results showed that diazepam increases the serum sodium, potassium, calcium, concentration significantly(p,0.01). It also increases the osmolarity; number of events and time spent in light box but decreased magnesium concentration significantly (P<0.01) in stressed and unstressed rats.

Conclusion: It is concluded that the diazepam reverse the stressed produced changes in serum electrolytes and plasma osmolarity.

Key words: Immobilization stress, hypertension, serum electrolytes and osmolarity.

For Full text contact to:
Prof. Dr. Farah Mansuri
editor@annals-ashkmdc.org

*Assistant Professor Biochemistry, KMDC, Karachi
**Professor of Biochemistry, Karachi University
***Professor of Biomedical Engineering, Sir Syed university of Engineering and Technology, Karachi