*SYED QAISER HUSSAIN NAQVI, **ANJUM NAQVI, ***MUHAMMAD ANWAR, ****MUHAMMAD SHERAZ KHAN, *****ANWAR ALI AKHUND.

ABSTRACT

Objective: To asses the frequency of HER-2/neu Oncoprotein over expression in invasive ductal breast cancer and correlate it with various prognostic parameters.

Methods: Comparitive cross-sectional study was carriedout at department of pathology Basic Medical Sciences Institute, Jinnah Postgraduate Medical Centre, Karachi. Seventy two(72) formalin fixed paraffin embedded blocks of diagnosed invasive ductal carcinoma were retrieved and 5 m.m thick sections were cut and stained with H&E for the review of diagnosis and grading. The immunohistochemical staining was done on 4m.m thick sections by using Polyclonal rabbit anti HER-2 ZYMED USA, and ZYMED 2nd generation LAB-SA immunodetection system, to see the HER-/neu over expression.

Results: Section containing > 50% of tumor cells exhibiting intense circumferential cell membrane staining scored as positive. Thirty one (31%) positive over expression was observed. A statistically significant result (P<.05) was found between HER-2/neu over-expression and lymph node status and size of tumor.

Conclusion: Oncoprotein HER-2 / neu over expression is directly related to the lymph node status and size of the tumor. These patients may benefit from a more aggressive therapy like Herceptin and can also be used as a prognostic marker in the follow up of these patients.

Key Words: Invasive ductal carcinoma. HER-2 / neu. Immunohistochemistry.

For Full text contact to:
Prof. Dr. Farah Mansuri
editor@annals-ashkmdc.org

*Assistant Professor, Department of Pathology, Peoples Medical College Nawabshah
**Assistant Professor, Department of Anatomy, Jinnah Post Graduate Medical Centre, Karachi
***Lecturer, Department of Pathology, Jinnah Post Graduate Medical Centre, Karachi
****Senior Demonstrater, Department of Pathology,
*****Professor Department of Pathology, Peoples Medical College Nawabshah